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PRPP Synthetase PowerPoint Presentation

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Description : A presentation on the characteristics of PRPP synthetase.

Tags : PRPP synthetase,PRPP,Phosphoribosylpyrophosphate,Phosphoribosylpyrophosphate synthetase,synthetase

Published on : Aug 15, 2022 | Views : 959 | Downloads : 0

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Slide 1-

GROUP 8 MEMBERS: Ntlokwana S. 202014856 Ndabetha A. 201927896 Latsha S. 202016076 Mtonga A. 202016684 Vatsha S. 202003708 Khalipha N. 201614708 Makeleni L. 202016082 Maqanda B. 202016787 Ntsikeni B. 202002676 Mhlanga S. V. 201704386 Properties of PRPP Synthetase
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IEC number The IEC number of PRPP synthetase is 2.7.6.1.
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PRIMARY SEQUENCE For class I of PRPP synthetase, the primary sequence contains two human PRPP synthetase subunits forming a homodimer, six SO4 2− ions and 183 water molecules in the asymmetric unit. Subunit A contains 305 amino acid residues from Asparagine to Phenylalanine except for Lysine to Valine, and subunit B contains 308 amino acid residues from Asparagine to Proline except for Arginine to Valine (Li et al., 2006).
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Functional 3D structure There are two human PRPP synthetase subunits (coloured in green and yellow respectively) in an asymmetric unit forming a homodimer (Li et al., 2006). The flexible loop involved in ATP binding is shown in blue, the PPi binding loop in magenta, the R5P binding loop in cyan and the flag region in red (for clarity, only one subunit is colour-coded). Figure 1: Structure of Human PRPP synthetase.
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Characteristics of its functional 3D structure Human PRPP synthetases (hPRS1) have three isoforms that share very high sequence identity (95.0% between hPRS1 and hPRS2; 94.3% between hPRS1 and hPRS3; and 91.2% between hPRS2 and hPRS3 respectively) (Li et al., 2006). Human PRPP synthetase 1 and hPRS2 genes are located on the X chromosome and are expressed in a wide range of tissues, but hPRS3 is an autosomal gene expressed specifically in testis (Li et al., 2006). Human PRPP synthetase 1 contains 318 amino acids and shares a moderate sequence identity with bacillus PRPP synthetase (bsPRS) (47% identity and 67% similarity respectively). It requires phosphate for activation and uses Mg2+ for activity (Li et al., 2006).
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PDB FILE PDB ID: 3 LRT
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ENZYME CLASS PRPP synthetase belongs to a class of enzymes that catalyse the transfer of specific functional groups from one molecule to another. This enzyme class is known as transferases (Becker, 2002).
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REACTION MECHANISM When observing the reaction below, the pyrophosphate group of ATP is transferred into ribose 5-phosphate, the product PRPP is a high-energy compound. PRPP synthetase has a soluble requirement for inorganic phosphate, which functions as an allosteric activator. The enzyme is inhibited by many nucleotides, of which are the product of the pathway for which PRPP is an essential substrate (Hove-Jensen et al., 2017). Figure 2: The reaction mechanism of PRPP synthetase.
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PATHWAY Pentose phosphate pathway Purine metabolism Metabolic pathways Biosynthesis of secondary metabolites Microbial metabolism in diverse environments Figure 3: Pentose phosphate pathway.
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LOCATION OF CELL It is located in the X-chromosomes.
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REFERENCES Becker, M.A. and Ahmed, M., 2002. Cell type-specific differential expression of human PRPP synthetase (PRPS) genes. Purine and Pyrimidine Metabolism in Man X, pp.5-10. Hove-Jensen, B., Andersen, K. R., Kilstrup, M., Martinussen, J., Switzer, R. L. and Willemoës, M., 2017. Phosphoribosyl diphosphate (PRPP): biosynthesis, enzymology, utilization, and metabolic significance. Microbiology and Molecular Biology Reviews, 81(1), pp.40-16. Iizasa, T., 2008. Increased activity of PRPP synthetase. Nihon rinsho. Japanese journal of clinical medicine, 66(4), pp.694-698. Li, S., Lu, Y., Peng, B. and Ding, J., 2006. Crystal structure of human phosphoribosylpyrophosphate synthetase 1 reveals a novel allosteric site. Biochemical Journal, 401(1), pp.39-47.
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Thank you!