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Polymerase Chain Reaction PowerPoint Presentation

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Description : POLYMERASE CHAIN REACTION

Tags : PCR,polymerase chain,chain reaction

Published on : Nov 20, 2022 | Views : 2039 | Downloads : 3

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Slide 1-

POLYMERASE CHAIN REACTION
Slide 2-

Contents
Slide 3-

Polymerase Chain Reaction
Slide 4-

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Slide 5-

Polymerase Chain Reaction
Slide 6-

PCR
Slide 7-

PCR Reaction Components
Slide 8-

PCR Reaction Components
Slide 9-

DNA Template
Slide 10-

Primers
Slide 11-

Four Normal Deoxynucleosides Triphosphate
Slide 12-

The standard PCR buffer contains:
Slide 13-

DNA Polymerase
Slide 14-

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Slide 15-

Tm= 2 C  X (number of A and T bases)+4 C  X (number of G and C bases).
Slide 16-

STANDARD PCR REACTION
Slide 17-

PCR
Slide 18-

Avoiding Contamination
Slide 19-

Sample Handling
Slide 20-

Laboratory Facilities
Slide 21-

Working with RNA
Slide 22-

Polymerase Chain Reaction
Slide 23-

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Slide 24-

Thermal Cycling Profile for Standard PCR
Slide 25-

Each cycle includes three successive steps:
Slide 26-

PCR
Slide 27-

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Slide 28-

Number of Cycles
Slide 29-

Gel Electrophoresis
Slide 30-

Gel Electrophoresis
Slide 31-

Gel Tray/ Loading
Slide 32-

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Slide 33-

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Slide 34-

PCR: Three Phases
Slide 35-

Polymerase Chain Reaction
Slide 36-

Variant of PCR
Slide 37-

Reverse Transcriptase-PCR
Slide 38-

RT-PCR
Slide 39-

Nested PCR
Slide 40-

Nested-PCR
Slide 41-

Hot-Start PCR
Slide 42-

Hot-Start PCR
Slide 43-

What is Wrong with Agarose Gels?
Slide 44-

Real Time PCR
Slide 45-

Real Time PCR
Slide 46-

Polymerase Chain Reaction: Uses
Slide 47-

Infectious Diseases/ Cancer
Slide 48-

Genetic Disease
Slide 49-

Inherited disorders diagnosed using PCR protocols
Slide 50-

Prenatal Diagnosis
Slide 51-

Research
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References & Further Reading
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About : Medical Doctorate Clinical Pathology Cairo University
Occupation : Medical Professional
Specialty : MD
Country : Egypt
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