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Electromyography PowerPoint Presentation

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Description : Check out this medical presentation on Neurology, which is titled "Electromyography", to know about electromyography and neurotransmitters.

Tags : Neurology, Electromyography, neurotransmitters

Published on : Aug 18, 2021 | Views : 2305 | Downloads : 10

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Slide 1-

Electromyography IE 665 Dr. Sengupta
Slide 2-

Outline Muscle Moment – Moment Arm Review of Muscle Contraction Physiology Physiological Basis of EMG Methods of EMG Collection Limitations & Uses (DHHS 91-100 chapter 2 and 5) Journal of Electromyography and Kinesiology (full-text in ScienceDirect)
Slide 3-

What is EMG Muscle contraction due to a change in the relative sliding of thread-like molecules or filaments Actin and Myosin Filament sliding is triggered by electrical phenomenon (ACTION POTENTIAL, AP) The recording of muscle APs is called electromyography (EMG) The record is known as an electromyogram
Slide 4-

Action Potential (AP) Cell membrane separates intracellular from extracellular space, diffusion barrier which restricts ION flow. Concentration of ions different inside vs. outside of cell membrane, results in an electrical potential difference known as a MEMBRANE POTENTIAL. Typical magnitude of membrane potential is -60 and -90 mV (interior of cell is negatively charged compared to the outside) when the muscle cell is in resting state. When sufficient neurotransmitters are deposited at the motor endplate, it opens up Na+ gates, causing an influx of Na+ ions, causing a rapid depolarization of the membrane near the motor end plate. The membrane potential can change to +20 to +50 mV at the motor endplate within a fractions of a second, which starts (all or none) a cascade of events.
Slide 5-

Action Potential (continued) http://upload.wikimedia.org/wikipedia/en/thumb/7/78/Apshoot.jpg/300px-Apshoot.jpg
Slide 6-

AP Continued This changed potential, sets up local currents in adjacent areas of membrane, which opens up more Na+ gates at the adjacent areas of the membrane, depolarizing those areas. The newly depolarized areas sets up electrical current with adjacent areas, and thus the depolarization wave propagates along the entire length of the muscle cell. Shortly after depolarization, the membrane is again repolarized, by active transport (ATP expenditure) of ions across the membrane. As a result a repolarization wave follows the depolarization wave for the entire length of the muscle.
Slide 7-

Action Potential Summary Active response of excitable membranes in nerve and muscle fibers produced by sodium and potassium channels opening in response to a stimulus AP abide by the all-or-none principle If MP reaches threshold voltage then Na+ channels open at first (Which direction will Na+ flow?) Na+ channels only open for 1 ms, this causes repolarization (K+ channels also open during this time to speed up return of resting membrane potential) AP propagation along muscle fibers AP propagation velocity dependent upon: (1) diameter of fibers (faster for thick – fast twitch) (2) [K+] in extracellular fluid, ie chemical environment.
Slide 8-

Motor Unit Action Potential Typically, each motorneuron innervates several hundred muscle fibers (innervation ratio) Motor Unit Action Potential (MUAP) = summed electrical activity of all muscle fibers activated within the motor unit Muscle force increased through higher recruitment and increased rate coding
Slide 9-

Recording Methodology Sweep of AP  similar to a wave Height of wave and the density of the wave can be recorded Represented graphically  electromyogram
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Recording Methodology (continued) Electrical potential difference measured between two points  bipolar electrode configuration used Bipolar Electrode Types Fine Wire Needle Surface Most common, less invasive Silver-silver chloride electrodes Electrode Placement Overlying the muscle of interest in the direction of predominant fiber direction Subject is GROUNDED by placing an electrode in an inactive region of body http://www.hhdev.psu.edu/atlab/EMG.jpg
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EMG Electrodes Fine wire Surface Electrodes Needle electrode
Slide 12-

Physiological Basis Hypothetical EMG from a single Muscle cell
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Physiological Basis Hypothetical EMG from a Motor unit
Slide 14-

Typical EMG recording Time axis (msec)  Amplitude (mv) Average amplitude over a time interval = 0
Slide 15-

Analyzing the EMG Signal Amplitude & Frequency More MU  more amplitude, more spikes and more turns in signal Change in firing rate  change in frequency content of EMG Change in muscle fiber type  change in AP velocity, change in EMG frequency. EMG is spatial and temporal summation of APs
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Average Rectified Amplitude Rectified = all negative values converted to positive values (absolute value) Then the average rectified amplitude provides a measure of signal strength
Slide 17-

EMG Amplitude vs Muscle Contraction Intensity Amplitude increases with increased contraction intensity, BUT it is not a linear relationship
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RMS Amplitude RMS (Root Mean Square): Each data point is squared and thus the negative values becomes positive. Mean is determined from the squared values Square root of this mean is RMS
Slide 19-

Factors Influencing Signal Measured Geometrical & Anatomical Factors Electrode size Electrode shape Electrode separation distance with respect to muscle tendon junctions Thickness of skin and subcutaneous fat Misalignment between electrodes and fiber alignment Physiological Factors Blood flow and temperature Type and level of contraction Muscle fiber conduction velocity Number of motor units (MU) Degree of MU synchronization
Slide 20-

Effect of electrode position on EMG
Slide 21-

Normalization Def: calibration against a known reference This allows researchers ability to compare different activities for the same muscle, different muscles, activities on different days, different subjects for same or different tasks, etc. Choices of normalization Maximum voluntary contraction (MVC) Functional activty Isometric activty Unresisted normal activity Submaximum contraction Limitations Variability of force generation due to motivation/physiological reasons
Slide 22-

What can be learned from an EMG? Time course of muscle contraction Contraction force Coordination of several muscles in a movement sequence These parameters are DERIVED from the amplitude, frequency, and change of these over time of the EMG signal Field of Ergonomics: from the EMG conclusions about muscle strain and the occurrence of muscular fatigue can be derived as well