Download Free Medical Diagnostic Cytopathology and Significance of Biopsy Investigation PowerPoint Presentation

Diagnostic Cytopathology and Significance of Biopsy Investigation PowerPoint Presentation

DOWNLOAD MEDICAL POWERPOINT PRESENTATION

About This Presentation

Description : Check out this medical presentation on Pathology, which is titled "Diagnostic Cytopathology & Significance of Biopsy Investigation", to know about diagnostic cytopathology and significance of biopsy investigation.

Tags : Pathology, Diagnostic Cytopathology, Significance, Biopsy Investigation

Published on : Aug 03, 2021 | Views : 1609 | Downloads : 1

Shareable Url :

Embed Code :

Comment

Transcript

Slide 1-

Diagnostic Cytopathology & Significance of Biopsy Investigation Jaroslava Dušková Inst. Pathol. ,1st Med. Faculty, Charles Univ. Prague https://www1.lf1.cuni.cz/~jdusk/
Slide 2-

Morphological Diagnostic Methods Clinical Pathological
Slide 3-

Morphological Diagnostic Methods Clinical: macroscopy of lesions visible with the naked eye invisible with the naked eye - IMAGING (X-ray, sonography, scintigraphy, endoscopy, CT,…) magnifying glass - colposcopy
Slide 4-

Morphological Diagnostic Methods Pathological macroscopy microscopy ultrastructure IMAGING
Slide 5-

Morphological Diagnostic Methods Pathological macroscopy of lesions autopsy report biopsy description cytology material description
Slide 6-

Morphological Diagnostic Methods Pathological microscopy cytology minibiopsy - cytoblock histology
Slide 7-

Cytology (FNAB) often both first and final dg. method outpatient low cost procedure done by an experienced (cyto)pathologist surprisingly effective has some limits (!)
Slide 8-

CYTOLOGY IS VERY EFFICIENT IN EARLY NEOPLASM DIAGNOSTICS
Slide 9-

ESPECIALLY, IF CLINICO-MORPHOLOGICAL COMMUNICATION WORKS
Slide 10-

Expectations Clinician from his pathologist: confirmation of neoplasm dg. nosological classification grading, staging prognosis reaction to the therapy recidive recognition
Slide 11-

Expectations Pathologist from his clinician: information: local.,size, duration, former dg. a treated neo, clin. dg. diagnostic material acquisition correct interpretation of the pathologist´s report
Slide 12-

Getting Cytology Material surface – smeared, brushed, scraped cavities – punctured, aspirated deep solid lesions - aspirated
Slide 13-

Processing Cytology Material smears cytospins, cytosedimentation cytoblock
Slide 14-

Minibiopsy - Cytoblock from FNAB advantage of easy material taking together with more tissue architecture information histology &, immunohistochemistry methods available multilayered tissue phragments readable
Slide 15-

Cytology Material Staining gynecology smears - polychrome other materials –MGG, HE, polychrome, all other methods cytoblock – multiple methods
Slide 16-

Goals of Cytological Investigation Screening – detection of symptomless lesions Diagnosis of pathological lesions found introductory (followed by histol.) final
Slide 17-

Gynecological oncologic cytology laboratory investigation standard Authors: MUDr Alena Beková, MIAC MUDr Pavel Tretiník, MIAC Oponents: doc. MUDr J. Dušková, CSc,FIAC MUDr Eva Svobodová
Slide 18-

Cytology - Evaluation staining and evaluation – minutes Bethesda system : material quality and quantity group diagnosis dg. as close to histology as possible recommendation
Slide 19-

Bethesda System 2001
Slide 20-

Suitable for Evaluation without limits limited by… non processed processed but limited for evaluation of squamous cell abnormalities due to…. .
Slide 21-

General Categorisation negative for intraepith. lesion or malignancy intraepith. lesion or malignancy squamous or glandular other pathology endometral cells in women over 40 yrs
Slide 22-

Interpretation negative for intraepithelial lesion or malignancy Microorganisms Trichomonas mycosis vs. candidosis shift - bact. vaginitis bacteria Actinomyces like cell changes of HSV type
Slide 23-

Interpretation negative for intraepithelial lesion or malignancy Other non-neoplastic changes reactive inflammation (+ repair) IUD atrophy
Slide 24-

Bethesda - cervical cytology classification 1. normal 2. benign cellular changes 3. ASCUS 4. L SIL 5. H SIL 6. Atypiae of glandular cells 7. susp. adenoca
Slide 25-

Interpretation epithelial abnormalities squamous ASC-US ASC-H LSIL CIN 1, HPV HSIL CIN2, CIN3, CIS susp. invasion squamous ca
Slide 26-

Interpretation epithelial abnormalities squamous ASC-US ASC-H LSIL CIN 1, HPV HSIL CIN2, CIN3, CIS susp. invasion squamous ca
Slide 27-

Bethesda System 2001
Slide 28-

Cytology getting sample needle 0.6-0.8mm min. 2 punctions aspiration nonaspiration – reduction of the blood content cyst: evacuate and aspirate with the second punction the periphery fluid: whole volume for cytology
Slide 29-

Peroperation Biopsy dg. during minutes morphological artefacts (combination with cytology) limited extensity of investigation limited time
Slide 30-

„Classical“ Biopsy (formol paraffin technique) simple may be done in two days immuno reactions two days more further sectioning two days more oncol. dg. - WHO classification typing, grading, staging prognostic factors
Slide 31-

Biopsy any tissue removed from a living patient is a COMPULSORY subject to do not follow the tendency to discard „unimportant“ materials (nevi, tonsillae, uteri, intestine, endometrial curretage…..) !
Slide 32-

Biopsy do not crush small samples taking imprint cytology !!! adequate amount of fixation solution (sample: fixative = min. 1:10) wide neck bottle tightly closed for transport but openable without rough violence flat sample – spread and tighten on a bearing socket mark discrete suspicious lesion with a stitch before fixation
Slide 33-

CYTOLOGYLOVE IT or LEAVE IT L. Cardozzo