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Bio Antimicrobial PowerPoint Presentation

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Description : e-Poster about the benefit of fish as bio antimicrobial

Tags : biology,bio antimicrobial

Published on : Oct 11, 2022 | Views : 703 | Downloads : 0

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REFERENCES Oroh SB. Kandou FEF. Pelealu J.Pandiangan D. 2015.Uji daya hambat ekstrak metanol Selaginella delicatula dan Diplazium dilatatum terhadap bakteri Staphylo- coccus aureus dan Escherichia coli. Jurnal Ilmiah Sains. 15(1): 52- 58. Patil RN, Kadam JS,Ingole JR, Sathe TV, Jadhav AD. 2015. Antibacterial activity of fish mucus from Clarias batrachus (Linn.) against selected microbes. Journal of Biolife. 3(4):788–91. Zakaria, Noer Khalid Chadir. 2015. Pengaruh Ekstrak Ikan Gabus (Channa striata) terhadap Penyembuhan Luka Pasca Operasi Bedah Laparatomi Kucing (Felis domestica). Jurnal Pustaka Kesehatan, 3(2), 195–198. The antimicrobial ability of mucus extract Chana striata (2.26 cm) is much stronger when compared to mucus Clarias batrachus (1.83 cm). But, both of these mucous are able to be used as antimicrobial agent. Mucus extracts of Clarias batrachus and Chana striata show antibacterial activity against the growth of E. coli fecal coliform bacteria originating from Gallus varius L’s feces. Future Work To optimize the usage of catfish and snakehead fish mucous, therefore future experiments would try to utilize these samples as an antimicrobial agent by identifying the other benefit compounds for making medicine, balms, and antiseptic soap. Chandra Kumala School ANTIMICROBIAL POTENTIAL OF CATFISH (Clarias batrachus) AND SNAKEHEAD FISH (Chana striata) ON BACTERIAL COLIFORM GROWTH IN CHICKEN FECES (Gallus gallus domesticus L.) Cherline, Jennifer, Vionita cherlinehalim06@gmail.com, jennifervcl25@gmail.com, vionita.itanza02@gmail.com Advised By Ms.Wenny Pintalitna, M.Pd wenny.tarigan@gmail.com This experimental study was carried out at the Chemistry and Biology Laboratory, Chandra Kumala School by isolating E.coli bacteria from native chicken feces using white NA Carolina media and culturing bacteria in differential EMB agar media. Next stage is bacterial purity test (Gram testing --> Gram negative; Citrate --> negative and Catalase Testing --> positive). Planting E.coli bacteria using the scratch method on EA media. The mucus extraction method used was a steeping method that was modified by soaking the skin and scales and then dried and pounded until smooth and then dissolved with 18% acetone for one day. Testing for antibacterial activity using the diffusion method of paper discs that were modified using filter paper soaked in an antibiotic solution (40% and 60% mucous extract, Cyprofloxacin; NaCl 0.9%) after being dry and the sterile affixed on to EA media. Inhibition zone measurement uses calipers. Calculating the effectiveness of mucous tested. Comparing the size of inhibition zone in every sample media Introduction Antibiotics are important drugs used in the treatment of bacterial infections. Repeated use of antibiotics in some bacteria causes resistance so that alternative therapies are needed by utilizing natural ingredients, one of which comes from fresh water. Research Goals This study aims to make antimicrobials from Clarias batrachus and Chana striata mucus which have the potential to inhabit the grow of coliform bacteria in Gallus varius L feces, and to determine the minimum inhibitory concentration of Clarias batrachus and Chana striata mucus extracts against Escherichia coli growth. Hypothesis Mucus extract of Clarias batrachus and Chana Striata have antimicrobial activity against the growth of E. coli. The minimum inhibitory concentration of Clarias batrachus and Chana striata mucus extracts on E. Coli growth was 40%. The results of this study indicated that the mucous Clarias batrachus and Chana striata at a concentration of 60% have shown antibacterial activity against the growth of E.coli faecal coliform bacteria originating in the feces of Gallus varius L. Isolating E.coli bacteria from feces 1 Culturing E.coli bacteria 2 Extracting the mucus from the fish (40% ; 60%) 3 Testing the sample into the agar media 4 Measuring the inhibition zone 5 Antimicrobial Mucus Product NA Carolina media EMB Agar media E.coli E.coli on EA media - + Gram Testing Catalase Testing Citrate Testing Feces extract Mucous extract Antibiotic paper disc Measurement of inhibitory zone E.coli on EA media NaCl 0.9% Cyprofloxacin 500 mg Mucous 40% Mucous 60% Table of Bacterial Inhibition Zone 1 2 3 Conclusion and Recommendation 6 7 8 Claricin, hepcidin, and protease enzymes as primary defense antimicrobial agents toward bacteria. Chana striata has albumin (6,224 gr / 100 gr) and protein (25.2%) which play a role in regulating blood osmotic pressure so that it can increase the ability of cells to fight bacteria. 4 Experimental Design 5 Data and Discussion E.coli on EA media NaCl 0.9% Cyprofloxacin 500 mg Mucous 40% Mucous 60%